19/03/2014 · SKBR3 cells (2 × 10 5 cells/ml) were seeded in 96well plate for 24 h. Medium was removed, and 100 μl/wells of plant isolates at various concentrations were added into each well and overnight incubated. Medium was removed and 50 μl of MTT solution (1 mg/ml) were added to each well and incubated at 37°C with 5% CO 2 for 2 h. After removal of ...